Rabbit Anti-PEDF抗體說明書

【資料簡介】

Rabbit Anti-PEDF抗體說明書
FROM: Abcam
Catalog Number :RS-0731R
Quantity size : 0.2ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）

Background: Pigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial （RPE） cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S （stressed） to R （relaxed） conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.

Rabbit Anti-PEDF抗體說明書
pecificity :
– Anti- PEDF is a rabbit monoclonal antibody unconjugated
– specific for PEDF of human, mouse, rat and pig
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 220kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Rabbit Anti-PEDF抗體說明書